How to Grow Mushrooms From Spores

Whether you’re a gardening enthusiast or a culinary adventurer, learning how to grow mushrooms from spores is a rewarding and fulfilling endeavor.

Mushrooms🍄 are a fascinating type of fungi that can be grown at home using spores. Cultivating mushrooms from spores allows you to have a fresh supply of these delicious and nutritious fungi right in your own garden or indoor space.

In this article📃, we will guide you through the process of how to grow mushrooms from spores, from selecting the right spores to creating optimal growing conditions.

How to grow mushrooms from spores

What Are Spores?

Before we delve into the process of growing mushrooms from spores, it’s important to understand what spores are.

Spores are the reproductive cells of fungi, similar to seeds in plants. They are tiny, single-celled structures that fungi use to reproduce and spread. Each spore has the potential to develop into a new mushroom under the right conditions.

How to Grow Mushrooms From Spores

To grow mushrooms from spores, you will need to create a suitable growing environment that mimics their natural conditions.

Here is a step-by-step guide on how to grow mushrooms from spores: 👇

Choosing the Right Mushroom Species

When it comes to growing mushrooms from spores, selecting the right mushroom species is crucial. Different mushroom species have specific requirements for growth and development.

Some popular mushroom species for cultivation include:

1. Button Mushroom (Agaricus bisporus): The button mushroom is one of the most widely cultivated mushrooms worldwide. It has a mild flavor and a firm texture, making it a versatile ingredient in various culinary dishes.

2. Oyster Mushroom (Pleurotus ostreatus): Oyster mushrooms are known for their delicate flavor and velvety texture. They come in various colors, including white, yellow, and pink. Oyster mushrooms are relatively easy to grow and are a great choice for beginners.

3. Shiitake Mushroom (Lentinula edodes): Shiitake mushrooms are highly prized for their rich, earthy flavor. They have a meaty texture and are commonly used in Asian cuisine. Shiitake mushrooms require specific growing conditions and are a popular choice among experienced cultivators.

When selecting a mushroom species, consider factors such as growing difficulty, climate, and personal taste preferences. It’s always a good idea to start with a species that is known for being beginner-friendly.

Creating the Ideal Growing Environment

Mushrooms thrive in specific environmental conditions, and creating the right growing environment is crucial for successful cultivation.

Here are some key factors to consider:

1. Light and Darkness:

Unlike plants, mushrooms do not require direct sunlight for growth. In fact, exposure to direct sunlight can be harmful to their development. Most mushrooms prefer low-light conditions or complete darkness.

A dark, temperature-controlled space such as a basement, closet, or dedicated grow tent is ideal for mushroom cultivation.

2. Temperature and Humidity:

Mushrooms have specific temperature and humidity requirements for optimal growth. The ideal temperature range for most mushroom species is between 65°F and 75°F (18°C and 24°C).

Maintaining a consistent humidity level of around 70% is crucial, as it mimics the natural conditions mushrooms thrive in. Using a hygrometer and a humidifier can help monitor and regulate humidity levels effectively.

3. Ventilation:

Proper airflow is essential in mushroom cultivation to prevent the growth of contaminants and ensure healthy mushroom development.

While mushrooms prefer high humidity, stagnant air can create a breeding ground for mold and other unwanted organisms. A gentle flow of fresh air can be achieved by using fans or by periodically opening the cultivation area.

By providing the right balance of darkness, temperature, humidity, and ventilation, you can create an environment where mushrooms can flourish.

Preparing the Substrate

The substrate is the material on which mushrooms grow and obtain their nutrients. Different mushroom species have different substrate preferences, but some common substrates include straw, wood chips, sawdust, and compost.

The substrate acts as a food source for the mycelium, the thread-like vegetative part of the mushroom.

To prepare the substrate, follow these steps:

1. Select and prepare the substrate material: Choose a substrate material suitable for your chosen mushroom species. If using a straw, soak it in water overnight and drain before use. For wood-based substrates, sterilize them to eliminate competing organisms.

2. Mix and hydrate the substrate: Combine the substrate material with water to achieve the desired moisture content. The substrate should be damp but not dripping wet. Proper hydration is crucial for mycelium growth.

3. Fill containers or bags: Pack the substrate into containers or bags, leaving some headspace for expansion. Seal the containers or bags to create a controlled and sterile environment.

Preparing the Substrate to grow mushrooms from spores

Inoculating the Substrate

Once the substrate is prepared, it’s time to introduce the mushroom spores into the substrate in a process called inoculation. This step involves creating an environment where the spores can germinate and develop into mycelium.

To inoculate the substrate, follow these steps:

1. Prepare a clean workspace: Cleanliness is vital during inoculation to prevent contamination. Disinfect the area and ensure you have all the necessary tools within reach.

2. Obtain mushroom spores: Purchase high-quality mushroom spore syringes or prints from a reliable supplier. Spore syringes are pre-filled with spore solution, while spore prints contain spores on a sterile medium.

3. Inject the spores: Using a sterile needle, inject the spore solution into the substrate material. Space the injections evenly to promote even colonization. For spore prints, scrape the spores onto the substrate surface.

4. Seal and incubate: After inoculation, seal the containers or bags to create a sterile environment. Place them in a warm and dark location for the mycelium to colonize the substrate. This process can take several weeks, depending on the mushroom species and environmental conditions.

Providing the Optimal Conditions for Growth

After inoculation, it’s crucial to provide the optimal conditions for the mycelium to grow and colonize the substrate fully.

Here are some key factors to consider during this stage:

1. Temperature:

Maintain the temperature within the desired range for your chosen mushroom species.

This stage often requires slightly higher temperatures than the initial substrate preparation stage to encourage mycelium growth. Monitor the temperature closely and make adjustments as necessary.

2. Moisture:

Maintaining proper moisture levels is essential for mycelium growth. Depending on the substrate and mushroom species, mist the substrate periodically to ensure it remains adequately hydrated.

Avoid oversaturating the substrate, as excessive moisture can lead to contamination.

3. Patience and Observation:

Growing mushrooms requires patience and careful observation. Monitor the growth progress regularly, looking for signs of healthy mycelium colonization and potential contamination.

Make adjustments as necessary to maintain the optimal growing conditions.

Maintaining Proper Hygiene

Maintaining proper hygiene throughout the mushroom cultivation process is crucial to prevent contamination and ensure successful growth.

Here are some hygiene practices to follow:

1. Sanitize equipment: Before and after each use, sanitize all tools, containers, and surfaces with a disinfectant solution. This prevents the introduction of unwanted organisms that could harm the mycelium.

2. Practice hand hygiene: Wash your hands thoroughly before handling any mushroom-related materials. Use gloves when necessary to prevent contamination from skin contact.

3. Control air quality: Keep the cultivation area clean and free from dust, mold spores, and other potential contaminants. Regularly clean the area and use air filters if needed.

4. Monitor and address contamination: If you notice any signs of contamination, such as mold growth or unusual odors, take immediate action to address the issue. Remove contaminated materials and adjust the growing conditions if necessary.

By maintaining proper hygiene practices, you minimize the risk of contamination and increase the chances of successful mushroom cultivation.

Also Read: How to Grow Mushrooms Indoors

Recognizing and Addressing Common Issues

Despite your best efforts, you may encounter some common issues during the mushroom-growing process.

Here are a few problems you might face and how to address them:


Contamination is one of the most common issues in mushroom cultivation. It can occur due to improper hygiene, poor substrate preparation, or exposure to airborne contaminants.

If you notice signs of contamination, such as unusual colors, foul smells, or mold growth, remove the affected materials immediately to prevent further spread.

Identify the source of contamination and make necessary adjustments to prevent recurrence.

Slow Growth

If your mushrooms are growing slower than expected, it could be due to suboptimal growing conditions or inadequate mycelium development.

Review the temperature, humidity, and moisture levels to ensure they are within the optimal range. Additionally, check the inoculation process and substrate quality.

Patience is key, as mushrooms can sometimes take longer to grow under less-than-ideal conditions.

Mushroom Diseases

Mushrooms are susceptible to various diseases, including bacterial infections and viral diseases. If you observe abnormal growth patterns, discoloration, or malformed mushrooms, it could be a sign of a disease.

Consult reliable resources or experts to diagnose and treat the specific disease affecting your mushrooms.

Remember that troubleshooting is an essential part of the learning process, and each challenge presents an opportunity to improve your cultivation techniques.

Also Read: Do Mushrooms Grow in Poop?

Harvesting and Storing Your Mushrooms

Congratulations! It’s time to reap the fruits of your labor and enjoy the bountiful harvest. Here are some guidelines for harvesting and storing your mushrooms:

Harvesting Mushrooms

1. Harvest at the right time: Different mushroom species have different indicators of maturity. Harvest the mushrooms when they reach their peak size but before the caps begin to open fully. This ensures optimal flavor, texture, and nutritional value.

2. Use a clean, sharp tool: When harvesting, use a clean knife or scissors to cut the mushrooms at the base of the stem. Avoid pulling or twisting, as this can damage the mycelium and the substrate.

3. Handle with care: Mushrooms are delicate and can bruise easily. Handle them gently to prevent damage and preserve their appearance.

4. Store properly: After harvesting, store the mushrooms in a breathable container such as a paper bag or a loosely sealed container. Place them in the refrigerator to extend their shelf life. Avoid storing them in airtight plastic bags, as this can promote moisture buildup and accelerate spoilage.

With proper harvesting and storage techniques, you can enjoy your homegrown mushrooms at their freshest and most flavorful.


Learning how to grow mushrooms from spores is a fascinating and rewarding journey.

By understanding the basics, choosing the right mushroom species, creating an ideal growing environment, and following proper cultivation techniques, you can cultivate your own delicious mushrooms right at home.

Embrace the process, be patient, and enjoy the magical world of mushroom cultivation!

Also Read: When to Harvest Oyster Mushrooms?


What are mushroom spores?

Mushroom spores are reproductive cells or seeds of fungi that are used to propagate and grow mushrooms.

Where can I get mushroom spores?

You can obtain mushroom spores from reputable online suppliers or specialty stores that sell mushroom cultivation supplies.

How do I prepare the growing substrate for mushroom spores?

The growing substrate can be prepared by mixing materials like straw, sawdust, or wood chips with water and nutrients, such as gypsum or bran, to create a suitable medium for mushroom growth.

Do I need any special equipment to grow mushrooms from spores?

While not essential, certain equipment like a clean workspace, containers for the substrate, a pressure cooker for sterilization, and a humidity-controlled environment can greatly improve your chances of success.

How long does it take for mushroom spores to grow into mushrooms?

The time it takes for mushroom spores to grow into mature mushrooms can vary depending on the species. Generally, it can take anywhere from a few weeks to several months.

How do I inoculate the substrate with mushroom spores?

The substrate can be inoculated by evenly distributing the mushroom spores onto the prepared medium and mixing them in thoroughly.

What are the ideal growing conditions for mushroom spores?

Most mushrooms prefer temperatures between 60-75°F (15-24°C) and a humidity level of around 80-90%. Additionally, they thrive in low-light conditions.

How do I maintain the required humidity for mushroom growth?

You can maintain humidity by misting the growing area with water, using a humidifier, or covering the growing containers with a plastic dome or lid.

How often should I water the mushroom substrate?

The substrate should be kept consistently moist but not overly saturated. Watering once or twice a day, depending on the moisture levels, is usually sufficient.

Can I grow mushrooms from spores indoors?

Yes, mushrooms can be successfully grown indoors using spores. Indoor cultivation provides better control over the growing environment.

How do I know when the mushrooms are ready to harvest?

Mushrooms are typically ready to harvest when the caps have fully opened and the gills underneath are visible. Harvesting them before the spores drop is recommended for better flavor and texture.

Can I reuse the substrate for growing more mushrooms?

Some substrates can be reused for multiple mushroom harvests, especially with species like oyster mushrooms. However, it is important to sterilize or pasteurize the substrate between batches to prevent contamination.

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